Blar i forfatter "Huser, Thomas Rolf"

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    • Cost-efficient nanoscopy reveals nanoscale architecture of liver cells and platelets 

      Mao, Hong; Diekmann, Robin; Liang, Hai; Cogger, Victoria Carroll; Le Couteur, David George; Lockwood, Glen P; Hunt, Nick; Schuttpelz, Mark; Huser, Thomas Rolf; Chen, Vivien; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-09)
      Single-molecule localization microscopy (SMLM) provides a powerful toolkit to specifically resolve intracellular structures on the nanometer scale, even approaching resolution classically reserved for electron microscopy (EM). Although instruments for SMLM are technically simple to implement, researchers tend to stick to commercial microscopes for SMLM implementations. Here we report the construction ...

    • High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components 

      Ortkrass, Henning; Schürstedt, Jasmin; Wiebusch, Gerd; Szafranska, Karolina Joanna; McCourt, Peter Anthony; Huser, Thomas Rolf (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-16)
      Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but the cost of the instruments is still relatively high, and the physical size ...

    • Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations 

      Mönkemöller, Viola; Øie, Cristina Ionica; Hubner, Wolfgang; Huser, Thomas Rolf; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2015)
      Liver sinusoidal endothelial cells (LSECs) act as a filter between blood and the hepatocytes. LSECs are highly fenestrated cells; they contain transcellular pores with diameters between 50 to 200 nm. The small sizes of the fenestrae have so far prohibited any functional analysis with standard and advanced light microscopy techniques. Only the advent of super-resolution optical fluorescence microscopy ...

    • New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations 

      Øie, Cristina Ionica; Mönkemöller, Viola; Hübner, Wolfgang; Schüttpelz, Mark; Mao, Hong; Ahluwalia, Balpreet Singh; Huser, Thomas Rolf; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-01-10)
      Super-resolution fluorescence microscopy, also known as nanoscopy, has provided us with a glimpse of future impacts on cell biology. Far-field optical nanoscopy allows, for the first time, the study of sub-cellular nanoscale biological structures in living cells, which in the past was limited to electron microscopy (EM) (in fixed/dehydrated) cells or tissues. Nanoscopy has particular utility in the ...

    • Optical trapping and propulsion of red blood cells on waveguide surfaces 

      Ahluwalia, Balpreet Singh; Mccourt, peter anthony; Huser, Thomas Rolf; Hellesø, Olav Gaute (Journal article; Tidsskriftartikkel; Peer reviewed, 2010)

    • Serial Raman spectroscopy of particles trapped on a waveguide 

      Løvhaugen, Pål; Ahluwalia, Balpreet Singh; Huser, Thomas Rolf; Hellesø, Olav Gaute (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      We demonstrate that Raman spectroscopy can be used to characterize and identify particles that are trapped and propelled along optical waveguides. To accomplish this, microscopic particles on a waveguide are moved along the waveguide and then individually addressed by a focused laser beam to obtain their characteristic Raman signature within 1 second acquisition time. The spectrum is used to distinguish ...